[关键词]
[摘要]
目的探讨小檗碱对胰岛素分泌缺陷型糖尿病大鼠肝脏葡萄糖激酶(GK)相关糖代谢的影响。方法观察小檗碱对实验性胰岛素分泌缺陷型糖尿病大鼠空腹血糖、血清胰岛素(INS)、糖原、肝脏乳酸(LA)、GK活性及其蛋白表达的影响。结果与模型组比较,小檗碱组大鼠血清空腹血糖水平明显降低,而INS、肝脏LA无明显变化,肝糖原合成增多、GK活性及其蛋白表达明显增高。结论小檗碱能调节胰岛素分泌缺陷型糖尿病大鼠糖代谢,这可能与其促进GK活性及其蛋白表达有关。
[Key word]
[Abstract]
Objective To study the effect of berberine on glucose metabolism in rats with insulin-secretion deficiency.Methods The fasting blood glucose (FBG) was observed before and after treatment, insulin (INS), hepatic glycogen,lactic acid (LA),and the activity of hepatic glucokinase (GK) were detected after two weeks of treatment.At the same time the protein expression of hepatic GK was determined using Western blotting. Results In comparison with the model control,the FBG level of berberine group was obviously lowered,and the levels of hepatic glycogen and GK significantly were raised,while the level of INS and hepatic LA showed no evident difference.Conclusion It is suggested that berberine has the significant effect on improving glucose metabolism in rats with insulin-secretion deficiency,which might be closely correlated with the effects on activating the activity and the expression of GK that is a key enzyme of glucose metabolism.
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[基金项目]
国家自然科学基金资助项目(30472254)