目的观察多罗清泰颗粒对人肝癌细胞株SMMC-7721信号转导基因表达的影响。方法体内抑瘤实验研究多罗清泰颗粒对小鼠肝癌移植瘤生长的影响;采用中药血清药理学方法和基因芯片技术,分析多罗清泰含药血清对人肝癌细胞株SMMC-7721信号转导基因表达的影响。结果多罗清泰颗粒能显著抑制小鼠肝癌移植瘤生长,抑制率为63%~67.8%。多罗清泰颗粒对所检测的99条信号转导基因中的68条基因表达具有显著影响,占所测基因的69%。其中,上调的基因为42条,下调的基因为36条。在多罗清泰颗粒小剂量处理组和大剂量处理组之间,SMMC-7721细胞信号转导基因表达则存在相当大的差异。结论多罗清泰颗粒能显著抑制肝癌细胞的生长,显著影响肝癌细胞信号转导基因的表达。

Objective To investigate the effect of Duoluoqingtai Granula (DLQTG) on gene expression in human hepatocellular carcinoma cell line (SMMC-7721).Methods Effect of DLQTG on the growth of mice-transplanted hepatocellular carcinoma was studied in vivo and on the expression of signal transduction genes of SMMC-7721 cell line with serum pharmacology and microarray. Results DLQTG was observed to significantly affect the growth of mice-transplanted hepatocellular carcinoma with inhibition rate from 63% to 67.8%,and the expression of signal transduction genes in 68 of 99 genes detected with up-regulation of 42 genes and down-regulation of 36 genes.There was obvious difference in the expression of signal transduction genes in SMMC-7721 cell line between low and high dosage of DLQTG-treated group.Conclusion DLQTG can be used to inhibit the growth of hepatocellular carcinoma and to affect the expression of signal transduction genes in human hepatocellular carcinoma significantly.