目的研究蓝萼甲素与大鼠血浆蛋白的结合。方法采用大鼠血浆平衡透析法,HPLC法测定透析袋两侧溶液中蓝萼甲素的质量浓度,计算蓝萼甲素的血浆蛋白结合率。结果蓝萼甲素在质量浓度为20、10、1μg/mL时与血浆蛋白结合率分别为74.46%、77.87%、75.29%。结论高效液相色谱法用于蓝萼甲素的生物样品测定具有简便、快速、灵敏与选择性高的特点。蓝萼甲素具有中等强度的血浆蛋白结合率。

Objective To study the protein binding rate of glaucocalyxin A in plasma of rat. Methods The plasma balance dialysis was used. An HPLC for the quantitative determination of glaucocalyxin A was presented and used to calculate the protein binding rate in plasma of rat. Results There were protein binding rate results of 74.46%, 77.87%, and 75.29% at three various concentrations 20, 10, and 1 μg/ mL of glaucocalyxin A in plasma of rat. Conclusion The HPLC method used to determine glaucocalyxin A is simple, rapid, and sensitive with good specificity, precision and accuracy, and glaucocalyxin A has medium capacities in protein binding rate in plasma of rat.

国家科技部863计划“创新药物和中药现代化”新药博士基金资助课题(2003AA2Z3518)