目的 通过体内骨髓细胞实验,探讨槟榔碱对DNA的损伤作用机制。方法 采用AO探针标记,激光共聚焦技术检测槟榔碱对骨髓细胞内DNA和RNA的影响;采用流式细胞仪测定槟榔碱对骨髓细胞周期的影响。结果 给予槟榔碱20、10、5mg/kg剂量的染毒组,骨髓细胞内RNA/DNA的荧光像素比值明显高于空白对照组,差异非常显著(P<0.01);骨髓细胞周期与空白对照组相比,G0/G1期细胞比率有非常显著增加(P<0.01),S期细胞比率有非常显著降低(P<0.01),G2/M期细胞比率有非常显著降低(P<0.01)。结论 槟榔碱对小鼠骨髓细胞的DNA有一定的损伤作用,具有一定的遗传毒性。

Objective? To study the mechanism of arecoline on damage to DNA through the bone marrow cell experiment in vivo. Methods? Laser Confocal Microscope was adopted to measure DNA and RNA of the bone marrow cells marked by AO probe. Influences of arecoline on cell cycle were observed by the Flow Cytometer. Results? Injection of arecoline to 20, 10, and 5 mg/kg posologic group, the fluorescence pixel ratio apparently higher than that in blank control group of the bone marrow cell inside RNA/DAN, the difference was significant (P0.01). Bone marrow cell cycle by comparison to blank control group, G0/G1 per cell ratio had got particularly increase (P0.01), S per cell ratio had got particularly decrease (P0.01), and G2/M per cell ratio also had decreased obviously (P0.01). Conclusion?? The arecoline have got definite damage action on DNA in bone marrow cell of mice and possessed some genetic toxicity.