[关键词]
[摘要]
目的建立三七剪口的HPLC指纹图谱,同时用于不同产地药材的皂苷成分定量。方法采用梯度洗脱法,对三七剪口水提物进行HPLC测定,流动相为水-乙腈,检测波长203nm;记录时间65min。结果运用梯度洗脱的方法能分离三七剪口的各类组分,并可对其中三七皂苷R1、人参皂苷Rg1、Re、Rb1和Rd进行定量。结论本实验建立的HPLC指纹图谱和测定方法可作为三七剪口有效组分的控制手段。
[Key word]
[Abstract]
To establish the HPLC fingerprint method of the rhizome of Panax notoginseng and set up a method for content determination of the main ginsenosides in the rhizome of P.notoginseng from various habitats.Methods HPLC Analysis was carried on C18 column with flow phase water-acetonitrile.The detection wavelength was at 203 nm,record time was 65 min.Results The componentscan be separated well and the ginsenosides Rg1,Re,Rbl,and Rd and notoginsenoside Rl can bedeterminated using this method.Conclusion The HPLC fingerprint of the rhizome of P.notoginseng andthe determination method which developed in this paper are suitable for the quality control of the rhizome of P.notoginseng.
[中图分类号]
[基金项目]
国家高技术研究发展计划(863计划)(2003AA2Z2021)