目的建立一套半夏工厂化育苗体系,实现半夏的快速繁殖,解决半夏生产因用种而造成的退化问题。方法探讨了外植体诱导、植物生长调节物质质量浓度、光照条件及继代周期对丛生芽增殖的影响。结果外植体的诱导以MS+6-BA(4~5)mg/L+NAA0.2mg/L培养基为宜。组织培养中生长调节物质质量浓度为NAA0.2mg/L+6-BA2mg/L、转接时组织块带两个或两个以上芽时,半夏生长状况最好。结论采用工厂化育苗技术可进行半夏的快速繁殖,为保护和持续利用这一重要药材资源提供有效途径。

Objective Industrialized breeding technology system will be established to achieve therapid multiplication of Pinellia ternata and to settle the problem of degeneration caused by seeds.MethodsExplant induction and effects of plant growth regulator dosage,illumination,and subculture cycle onfaseiculated shoot propagation were studied.Results It is good for the explant induction on mediumMS+6-BA(4-5)mg/L+NAA O.2 mg/L.P.ternata grows well under the circumstance of NAA0.2 mg/L+6-BA 2 mg/L,double or more shoots.Conclusion Industrialized breeding technology systemof P.ternata can make its propagation rapid and support an effective way for resources preserving andsustainable utilization.

重庆市科技计划项目(8024)