目的研究培养基种类、盐强度和有机物对丹参Salvia miltiorrhiza不定根生长及其丹参酮A和原儿茶醛合成的影响。方法利用组织培养技术结合HPLC手段,考察培养基种类、盐强度和有机物对丹参不定根生长和丹参酮ⅡA、原儿茶醛量的影响。结果通过考察MS、LS、B5、White和SH培养基对丹参不定根培养的影响,综合考虑不定根生长速率和有效成分的量,确定MS为基本培养基。改变培养基盐强度发现,高盐强度利于不定根生长,低盐强度促进丹参酮ⅡA和原儿茶醛合成。MS培养基5种有机物之间的相互作用对丹参不定根生长起很大作用;甘氨酸有利于丹参酮ⅡA的合成;仅缺少肌醇、甘氨酸、VB1、VB6其中一种时,原儿茶醛合成均受阻。结论确定了丹参不定根悬浮培养的基本培养基为MS,培养基盐强度和有机物处理对丹参不定根生长和次生代谢物合成有显著影响。

Objective To study the effects of various media,salt intensity,and organic components on the growth of Salvia miltiorrhiza adventitious roots and the synthesis of tanshinone I A and protoca-techuic aldehyde.Methods The adventitious roots were obtained through tissue culture by manipulationof various media,salt intensity,and organic components and the contents of tanshinone I A andprotocatechuic aldehyde were determined by HPLC.Results The effect of media MS,LS,B5,White,and SH on adventitious roots of S.miltioiza was observed.Adventitious roots grew betterunder highsalt intensity while secondary metabolite biosynthesis Wfl8 accelerated under 10W salt intensity in MS basal medium.The reciprocity of five organic components had significant effect on root growth #glycin favoredthe synthesis of tanshinone i A；scarcity of one of inosital,glycin,VBl,and VB6 inhibited the synthesis ofprotocatechuic aldehyde.Conclusion MS Basal medium iS used for adventitious root culture.The Resultsshow that salt intensity and organic components have significant effects on adventitious root culture of S.miltiorrhiza and secondary melabolite synthesis.

国家863项目(2003AA2Z2040);国家中医药管理局中医药科学技术研究专项(04-05ZP10)