目的观察丹参脂溶性有效部位对吗啡诱导的小鼠条件性位置偏爱效应的影响,并对丹参有效部位进行初步鉴定。方法隔天分别sc吗啡和生理盐水,共6d,引起小鼠产生显著的条件性位置偏爱效应。在训练阶段sc吗啡前30minip不同剂量的丹参脂溶性有效部位。建立反相高效液相色谱法(RP-HPLC)鉴定丹参脂溶性有效部位的主要成分。结果吗啡模型组小鼠在伴药箱停留时间明显延长,丹参脂溶性有效部位(40mg/kg)可使小鼠在伴药箱停留时间显著缩短(P<0.01)。经RP-HPLC法初步鉴定丹参脂溶性有效部位的主要成分为隐丹参酮。结论丹参脂溶性有效部位(隐丹参酮)能在一定程度上抑制吗啡诱导的小鼠条件性位置偏爱效应的形成。

Objective To investigate the effect of lipid soluble fraction in the radix of Salvia miltiorrhiza(FSM)on conditioned place preference(CPP)in mice induced by morphine and preli minarilyidentify the fraction in the radix of S.miltiorrhiza.Methods Morphine or NS was sc injected every otherday to induce the obvious CPP in mice for 6 d.Before 30 min of sc injecting morphine.mice were iPadministered different doses of lipid soluble fraction in the radix of S.miltiorrhiza.RP-HPLC method wasused to identify the major component in the lipid soluble fraction in the radix of S.miltiorrhiza.Results The staying time in morphine-paired white compartment was significantly prolonged.After treatment withIipid soluble fraction in the radix of S.miltiorrhiza(40 mg/kg,ip),the staying time in morphi ne-pairedwhite compartment was significantly shortened(P<0.01).The major component in the lipid solublefraction of S.miltior'rhiza was indentifed as crypt otanshinone.Conclusio n Lipid soluble fraction in theradix of S. miltiorrhiza (crypt otansh inone) could restrain the acquisition of CPP in mice induced bymorphine to a certain extent.

福建省自然科学基金资助项目(04A026)