目的探讨补阳还五汤对大鼠局灶性脑缺血后血管内皮生长因子(VEGF)及其受体(Fetal liver kinase1,Flk1)的影响。方法大鼠随机分为假手术组、模型组、尼莫地平组、补阳还五汤组,大脑中动脉线栓法复制局灶性脑缺血模型,采用免疫组织化学法和酶联免疫法检测各组动物脑内VEGF、Flk1的表达和蛋白水平,同时评价动物神经功能。结果在正常大鼠脑内有少量VEGF和Flk1阳性细胞,脑缺血后VEGF和Flk1阳性细胞增加;与模型组比较,尼莫地平、补阳还五汤均能改善模型大鼠的神经功能缺失症状,增强VEGF和Flk1的表达,提高VEGF蛋白水平(P<0.05);于7、14d补阳还五汤作用强于尼莫地平(P<0.05)。结论补阳还五汤增强大鼠局灶性脑缺血后VEGF及Flk1的表达和提高VEGF蛋白水平,是其抗脑缺血的可能作用机制之一。

Objective To explore the effect of Buyang Huanwu Decoction(BYHWD)011 vascular endothelial growth factor(VEGF)and itS receptor,Fetal liver kinase 1(Flk 1)in rats after focal cerebralischemia.Methods Rats were randomly divided into following groups including Sham group,modelgroup,Nimodipine group,and BYHWD group.The model of focal cerebral ischemia in rats wasreproduced by middle cerebral artery occlusion.Rats were ig administered and killed after operated,then theexpression of VEGF and Flk l and protein level of VEGF in brain tissue of every groups were measured byimmunohistochemisty and enzyme linked immunosorbent assay,and the nervous function deficit scoreswere evaluated.Results There were a few VEGF and Flkl positive cells in normalbrain tissue of rats.After focal cerebralischemia the VEGF and Flk l positive cells were increased.Compared to model group.Nimodipine and BYHWD significantly improved the neurological behavior performance.increased thenumbers of VEGF and Flk l positive cells,and enhanced the VEGF protein level(P<0.05).There weremore potential effect of BYHWD than Nimodipine (PConclusion BYHWD can enhance the VEGF expressions and Flk l level in rats after focal cerebral ischemia.which iSone of possible anti-ischemic mechanism of BYHWD.

国家自然科学基金资助项目(30300470,30472217);湖南省教育厅优秀青年项目(04B046)