[关键词]
[摘要]
目的建立决明子饮片的HPLC指纹图谱鉴定方法。方法采用RP-HPLC法,梯度洗脱,研究决明子饮片的指纹图谱,并作相似度比较分析。结果初步建立了决明子饮片的指纹图谱。结论采用HPLC指纹图谱法可作为决明子饮片的一项质控指标。
[Key word]
[Abstract]
Objective To establish HPLC fingerprint for the identification of Semen Cassiae.MethodsChromatographic fingerprint of Semen Cassiae was investigated by RP-HPLC and the gradient elution modewas applied to chromatographic separation.Data were analysed by fingerprint similarity evaluationsoftware to compare the similarity of the samples.Results The HPLC fingerprint of Semen Cassiae wasestablished preliminarily.Conclusion HPLC Fingerprint method is repeatable and can be used in quality control of Semen Cassiae.
[中图分类号]
[基金项目]
国家“十五”重大科技专项“创新药物和中药现代化”课题(2001BA701A55-41)