目的探讨对西兰花中葡萄糖异硫氰酸盐(glucosinolates,GS)诱导人胃腺癌SGC-7901细胞凋亡的作用及其可能机制。方法不同质量浓度GS处理人胃腺癌SGC-7901细胞,通过SRB法、细胞形态学观察、流式细胞仪和激光共聚焦显微镜实验,观察GS对SGC-7901细胞的抑制率,对细胞凋亡和细胞周期的影响。结果1、10、100、1000μg/mL的GS作用于SGC-7901细胞72h后,抑制了SGC-7901细胞的增殖,其IC50为187.723μg/mL;300μg/mL的GS作用SGC-7901细胞24h后,细胞出现早期凋亡的形态;300、600、1200μg/mL的GS作用于SGC-7901细胞24h后,可见细胞凋亡率分别为(14.54±6.69)%、(10.11±6.25)%、(34.12±13.29)%,并且G2期细胞数目减少至0,对细胞周期有影响;100、200、300μg/mL的GS作用于SGC-7901细胞24h后,细胞内的Ca²⁺浓度升高,且随着GS给药剂量的增加而升高。结论GS能够促进人胃腺癌SGC-7901细胞凋亡,影响细胞周期,此作用可能是GS升高细胞内Ca²⁺的浓度而达到的。

Objective To investigate the effect of glucosinolates (GS) in broccoli on apoptosis of human gastric adenoma cells SGC-7901 and its possible mechanism. Methods SGC-7901 cells were treated with different concentrations of GS. SRB Assay, fluorescence microscope, flow cytometry, and laser confocal microscopy were used to observe the inhibitory rate, apoptosis rate, and cell cycle of SGC-7901 cells by GS. Results 1, 10, 100, and 1 000 μg/mL of GS affected on SGC-7901 cells for 72 h, GS inhibited proliferation of SGC-7901 cells, and its IC₅₀ was 187.723 μg/mL; 300 μg/mL of GS affected on SGC-7901 cells for 24 h, cells appeared the modality of early apoptosis; 300, 600, and 1 200 μg/mL of GS affected on SGC-7901 cells for 24 h, the apoptosis rates were (14.54±6.69)%, (10.11±6.25)%, and (34.12±13.29)%, and the number of G2 cycle cells decreased to zero; 100, 200, and 300 μg/mL of GS affected on SGC-7901 cell for 24 h, the variation of Ca²⁺ in cells was increased, and it can be raised with the increase of dose. Conclusion GS could promote the apoptosis of SGC-7901 cells and affect cell cycle, its mechanism may be to increase the Ca²⁺ in cells.

国家自然科学基金项目(30300284,30400592)；黑龙江省自然科学基金重点项目(ZJY03—04)；哈尔滨市科技攻关项目(2004AA9CN164)