目的建立HPLC法测定不同产地、不同栽培品系的罗汉果药材中罗汉果苷V和11-氧化罗汉果苷V。方法采用ODS(250mm×4.6mm,5μm)色谱柱,以乙腈-水为流动相进行梯度洗脱,体积流量0.75mL/min,检测波长210nm,柱温40℃。结果罗汉果苷V在0.8046~20.1150μg线性关系良好(r=0.9998),平均回收率为104.6%,RSD为3.28%(n=6)。11-氧化罗汉果苷V在0.5985~14.9625μg线性关系良好(r=0.9984)。结论本方法简便、快速、准确,可作为罗汉果药材的质量控制方法。

Objective To establish an optimal HPLC method for determining the contents of mogro-side V and 11-oxomogroside V in the dried mature fruits of Momordicae grosvenori from various habitats and breeds. Methods ODS Column (250 mm× 4.6 mm, 5 μm) was used with acetonitrile-water as mobile phase in a gradient program, flow rate was 0.75 mL/min, detection wavelength was set at 210 nm, column temperature was 40℃. Results The liner range of mogroside V was 0.804 6-20.115 0 μg (r=0.999 8 ) and the average recovery of mogroside V was 104.6%, RSD was 3.28% (n=6). The liner range of 11-oxomogroside V was 0.598 5—14.962 5 μg (r=0.998 4) and the average recovery was 102.5%, RSD was 4.43% (n=6). Conclusion HPLC method is simple, rapid and accurate. It can be used for the quality control of the dried mature fruits of M. grosvenori.

国家“863”资助项目(2004AA2Z3730-19)