[关键词]
[摘要]
目的从灵芝Ganoderma lucidum子实体中分离得到的GL-PPT2、GL-PPT3和GL-PPT43种多糖肽,对其单糖组成和摩尔比进行比较。方法多糖肽经三氟乙酸水解后,用1-苯基-3-甲基-5-吡啶啉酮(PMP)衍生化,在高效液相245nm紫外检测,使用KH2PO4(pH5.0)-乙腈(83.5∶16.5)等度洗脱,9种单糖的色谱峰分离清晰,大多呈基线分离。结果经实验确定GL-PPT2、GL-PPT3和GL-PPT4均由甘露糖、核糖、鼠李糖、葡萄糖、半乳糖、阿拉伯糖、木糖、岩藻糖和葡萄糖醛酸等9种单糖组成。结论灵芝子实体活性成分多糖肽中的核糖系属首次报道。
[Key word]
[Abstract]
Objective To compare the monosaccharide composition and molar ratio of GL-PPT2, GL-PPT3, and GL-PPT4, which are three polysaccharide peptides isolated from the fruit body of Ganoderma lucidum. Methods The polysaccharide peptides were hydrolyzed by triflouroacetic acid, then derivatized by trichloro-aldehyde-1-pheny-3-methyl-5-pyrazolone (PMP), and determined by HPLC method with the 245 nm detection wavelength. Gradient elution with the solution of KH2PO4 (pH 5.0)-acetonitrile (83.5∶16.5) resulted in baseline separation of nine monosaccharides, of which the chromatographic peaks were very clear. Results GL-PPT2, GL-PPT3, and GL-PPT4 comprise the nine monosaccharides of mannose, ribose, rhamnose, glucose, galactose, arabinose, xylose, galactose, and glucuronic acid. Conclusion The ribose in polysaccharide peptides of G. lucidum is first reported.
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[基金项目]
上海绿谷集团有限公司科研基金资助项目