目的研究β1整合素在淋巴瘤中的表达及姜黄素对其的影响。方法免疫组化法检测B细胞淋巴瘤、T细胞淋巴瘤和反应性增生淋巴结中β1整合素表达的差异;流式细胞术检测Raji细胞、Daudi细胞和正常人外周血淋巴细胞β1整合素表达的差异;流式细胞术和RT-PCR方法检测姜黄素对Raji细胞β1整合素mRNA和蛋白水平的影响。结果β1整合素在Raji细胞中高表达,Daudi细胞及正常B淋巴细胞中β1整合素表达水平很低;T细胞淋巴瘤淋巴结和反应性增生淋巴结β1整合素表达显著低于B细胞淋巴瘤淋巴结;低浓度姜黄素即能显著抑制β1整合素的表达。结论β1整合素在Raji细胞中高表达,在正常人淋巴结和外周血淋巴细胞中低表达,且β1整合素在不同类型淋巴瘤中的作用不同,姜黄素能显著抑制β1整合素的表达,有希望成为抑制淋巴瘤侵袭、转移的新药。

ObjectiveTo investigate the expression of β1 integrin in lymphoma and modulatory effect of curcumin on it. Methods Immunohistochemistry was used to detect the difference of β1 integrin expression in B-lymphoma, T-lymphoma, and reactive hyperplasia lymph nodes. Flow cytometry was used to detect the difference of β1 integrin expression in Raji cells, Daudi cells, and lymph cells in normal human peripheral blood. Flow cytometry and reverse transcriptase-polymerase chain reaction (RT-PCR) methods were used to determine the effect of curcumin on β1 integrin mRNA and protein level of Raji cells. Results β1 Integrin was highly expressed in Raji cells but lowly expressed in Daudi cells and normal B lymphocyte. β1 Integrin expression in lymph node of T lymphoma and reactive hyperplasia lymph node were significantly lower than that in lymph node of B lymphoma. Curcumin at low concentration can markedly inhibit the expression of β1 integrin. Conclusion β1 Integrin can be highly expressed in Raji cells but lowly expressed in normal human lymph nodes and lymphocyte of peripheral blood. The expression level of β1 integrin in various types of lymphoma is different. Curcumin can markedly inhibit the expression of β1 integrin and may be a new drug which can inhibit the invasion and metastasis of lymphoma

国家自然科学基金资助项目(30472267)