目的探索电致孔条件下不同电学参数(电压、波形、脉冲宽度、脉冲次数)对青藤碱透皮给药的影响。方法用中药电致孔给药仪产生多种电学参数条件,并以双室扩散池法、HPLC法检测青藤碱透过小鼠腹部皮肤的变化。结果青藤碱的累积透过量与电参数密切相关,在单因素影响中,4个最佳电学参数分别为600V电压、正弦波+方波组合波形、500μs的脉冲宽度、240个脉冲次数。透过量有极显著的增大,与被动扩散相比增加幅度有5~100倍。结论优化的电致孔条件应用于中药有效成分透皮给药有良好的前景。

Objective To observe the effects of different electricalparameters(voltage，shapof wave，pulsewidth，and number ofpulses)ontransdermaldeliveryof sinomenineusingeletroporation．MethodsThe transdermalpenetrationin Franz diffusion celI for isolatedratskin of sinomenine was studiedbydif-ferentelectricalparametersofelectroporation equipmentfor Chinese materiamedica，respectively．Thecontentofsinomenineper-meatedabdomen skin of mice was determined bv HPI。C．Results The accumula-tivepermeationof sinomenine was correlated with electricalparameters。differentelectricalparametersshowed thesignificanteffect on sinomenine’S transdermaldelivery．The optimumelectricalparameterswerevoltage：600 V，shapof wave：the combination ofsquarewave and sinewave，pulse width：500弘s，number ofpulses：240．The permeationincreasedsignificantlyand theincreasingextentwas 5--1 07 timescomparedwith thepassivediffusion．ConclusionOptimum parametersofeletroporationhave an favorablefuturefor active constituents in Chinese materia medica to betransdermicallydeliveried