目的采用特异引物PCR扩增鉴定马鹿和梅花鹿源中药材。方法从马鹿、梅花鹿等国内11种鹿的鹿血和鹿茸中提取DNA,用通用引物L1091和H1478调取细胞线粒体12SrRNA基因片段,在该片段核苷酸序列比对的基础上,设计马鹿和梅花鹿高特异性PCR鉴定引物对,并进行PCR特异性鉴定。结果12SrRNA基因片段能较好地在种的水平上将不同的种区分开来;特异引物对(EP-1/H1478和EP-2/H1478)PCR可准确地鉴定出马鹿和梅花鹿等。结论特异引物PCR适宜马鹿、梅花鹿等珍贵中药材的鉴定。

Objective To identify the animal drug of Cervus elaphs and C. nippon from origin of deers. Methods To extract DNA from deer blood and hairy antler of 11 species of deers such as C. elaphus, C. nippon and so on, and to gain the mitochondrial 12S rRNA gene fragment using the general primers of L1091 and H1478. Based on the sequence multialinement of 11 species deers above gene fragments, designing the couples of special difference primers and identifying C. elaphs and C. nippon. Results 12S rRNA Gene fragments can distinguish different deers well. The couple of primers (EP-1/H1478 and EP-2/H1478) PCR can effectively identify C. elaphus and C. nippon. Conclusion Special primer PCR is suitable for the identification of valuable Chinese medicinal materials, such as C. elaphus and C. nippon.

北京中医药大学211工程项目资助