目的比较复方五仁醇胶囊主要入血成分在大鼠血清和血浆中的量。方法灌胃给药制备大鼠含药血清和含药血浆,HPLC法测定五味子醇甲和五味子乙素。LichrosphereC18(250mm×4.6mm,5μm)色谱柱,Phe-nomenexDescriptionC18保护柱(4.0mm×3.0mm),甲醇-水梯度洗脱,体积流量1.0mL/min,柱温30℃,检测波长254nm。结果五味子醇甲、五味子乙素分别在0.0512~0.6144和0.0398~0.4776μg线性关系良好,低、中、高3个水平五味子醇甲、五味子乙素的平均相对回收率分别为96.72%、101.06%、102.05%和99.03%、100.18%、100.28%。血清和血浆中五味子醇甲平均质量浓度分别为11.0632、12.8837μg/mL,五味子乙素平均质量浓度分别为7.4909、12.5908μg/mL。结论复方五仁醇胶囊主要入血成分血浆中的量高于血清。

Objective To compare the content of main constituents of Compound Wurenchun Capsules dissolved in serum and plasma of rats. Methods Serum and plasma containing drug were prepared after ig the preparation to rats. Lichrosphere C_ 18 (250 mm×4.6 mm, 5 μm) column and Phenomenex Description C_ 18 (4.0 mm×3.0 mm) protective column were used. The mobile phase consisted of methanol-water, eluted in gradient mode. The flow rate was 1.0 mL/min. The column temperature was 30 ℃ and detection wavelength was 254 nm. Results The linear ranges of schisandrin and schisandrin B were 0.051 2 .614 4 and 0.039 8—0.477 6 μg. The average relative recoveries of schinsandrin and schisandrin B were 96.72%, 101.06%, 102.05%, and 99.03%, 100.18%, 100.28% in low, middle, and high concentrations, respectively. The average contents of schisandrin in serum and plasma were 11.063 2 and 12.883 7 μg/mL, schisandrin B were 7.490 9 and 12.590 8 μg/mL, respectively. Conclusion The main constitu-ents of Compound Wurenchun Capsules contained in plasma account for higher than the ones in serum.

江苏省中医药局中医药科学技术研究专项课题(H05173);南通市社会发展科技计划项目(S4006)