[关键词]
[摘要]
目的建立苦瓜子蛋白分离纯化的方法。方法应用阳离子交换色谱和凝胶过滤色谱Hiprep16/10SOURCE30S和HiLoad26/60Superdex75预装柱从苦瓜种仁中分离纯化苦瓜子蛋白。结果分离纯化出14个组分的苦瓜子蛋白,它们均为碱性蛋白质,相对分子质量在(1.3~2.9)×104,等电点在9.3~9.6。结论应用本法能获得供生物活性研究用苦瓜子蛋白组分。
[Key word]
[Abstract]
Objective A method was developed for the isolation and purification of proteins in the seeds of Momordica charantia. Methods The proteins were isolated and purified from the seeds of M. charantia by a procedure involving extracting with acetones and a series of chromatographies on Hiprep 16/10 SOURCE 30S and HiLoad 26/60 Superdex 75. Results Fourteen protein fractions were purified from the seeds of M. charantia. They are all basic proteins with the molecule weight range (1.3-2.9)×10~4 and isoelectric point range 9.3-9.6. Conclusion The bioactive proteins can be purified from the seeds of M. charantia by a procedure involving extracting with acetone and a series of chromatographies on Hiprep 16/10 SOURCE 30S and HiLoad 26/60 Superdex 75.
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[基金项目]
广东省教育厅自然科学研究项目(Z02041)