目的研究补阳还五汤及其有效部位生物碱、苷对大鼠脑缺血再灌注后脑组织caspase-1、3、8表达的影响。方法采用颈内动脉线栓法建立大鼠大脑中动脉阻塞(M CAO)再灌注模型，以逆转录-聚合酶链反应(RT-PCR)测定缺血脑组织中caspase-1、3、8 mRNA的表达。结果脑缺血2 h再灌注22 h后，模型组caspase-1、3、8表达较假手术组均显著增强(P<0.05)。生物碱组、苷组caspase-1表达显著低于模型组(P<0.05)；补阳还五汤组、生物碱组、苷组、三七总皂苷组caspase-3与模型组比较均显著降低(P<0.05)；生物碱组caspase-8表达较模型组显著降低(P<0.05)。三七总皂苷组caspase-8表达较假手术组显著增强(P<0.05)。结论补阳还五汤及其有效部位通过抑制缺血后脑组织caspase-1、3、8的表达，从而有可能对抗脑缺血再灌注后神经细胞凋亡，保护缺血脑组织。

Objective To investigate the effects of Buyang Huanwu Decoction (BHD) and two kinds of fractions extracted from BHD on expression of caspase-1, caspase-3, and caspase-8 mRNA after cerebral ischemia-reperfusion in rats. Methods The model of cerebral ischemia-reperfusion was induced by the middle cerebral artery occlusion (MCAO) in rats, via string ligation of arteria carotis interns. The expreslion of caspase-1, caspase-3, and caspase-8 mRNA in cerebral ischemic tissues was assessed by reverse transcription-polymerase chain reaction (RT-PCR). Results The expression of caspase-1, caspase-3, and caspase-8 mRNA in cerebral tissues was significantly increased after 22h reperfusion following 2h cerebral ischemia in model group (P<0.05). Compared with model group, the expression of caspase-1 was decreased remarkably in alkaloid group and glycoside group (P<0. 05).BHD, alkaloid, glycoside, and Panax notoginseng saponins (PNS) could reduce the expression of caspase-3 significantly (P<0. 05). The expression of caspase-8 in alkaloid group was decreased compared with the model group (P<0. 05). The expression of caspase-8 mRNA in PNS was enhanced when compared with Sham-operated group (P<0.05). Conclusion BHD, alkaloid, and glycoside can inhibit the expression of caspase-1, caspase-3, and caspase-8 mRNA after cerebral ischemia-reperfusion injury, then antagonize the nervous cell apoptosis and protect the cerebral ischemic tissues.

国家自然科学基金项目(30171132);教育部高等学校学术与技术带头人资助计划项目;湖南省优秀博士论文作者科研项目