目的通过测定在不同的萃取条件下橘红中重金属的量，探讨这些条件对超临界CO₂萃取橘红中重金属的影响。方法以二乙基二硫代氨基甲酸钠（N aDDC3H₂O）作为配合剂，乙醇为夹带剂，设计正交试验，采用ICP-M S测定橘红中Cu、As、Pb的量。考察了萃取压力、温度、配合剂和萃取时间等条件。结果在样品为10g时，萃取压力25MPa，温度60℃，配合剂NaDDC用量2g，萃取时间3h，夹带剂乙醇用量10mL，萃取后重金属的量可达到美国FDA标准。结论萃取后的药材中重金属量显著下降，为降低中药材中重金属开辟了一条新的思路和研究方法。

Objective To make an attempt at some conditions on extracting heavy metals in chrysoidine by supercritical CO₂ extration. Methods Taking sodium diethyldithiocarbamate trihydrate(NaDDC·3H₂O) as chelating agent and ethanol as entrainer, the orthogonal test was designed and ICPMS used to mensurate the contents of Cu, As and Pb in chrysoidine under different conditions. According to the results, the factors including extracting pressure, temperature, chelating agent dosage, and time were studied. Results When the test sample was 10g, the optimum condition was that extracting pressure:25MPa, the temperature:60℃, chelating agent dosage:2g, extracting time:3h, and the ethanol dosage:10mL. After extracting reaction, the contents of heavy metal in chrysoidine reached United States FDA standard. ConclusionThe contents of heavy metal in Chinese herb medicine are notably decreased, and this way provides a new thought and research technique to decrease the contents of heavy metal.

教育部高等院校博士点基金资助(20020561026);广东省自然科学基金重点项目(036556)