目的观察和比较人、大鼠、Beagle犬及家兔肠道菌群对菊花提取物（Chrysanthemum morifolium extracts,CME）的代谢作用。方法采用人、大鼠、Beagle犬及家兔的新鲜粪便37℃厌氧培养，经醋酸乙酯提取后采用RP-HPLC及LC-MS方法对代谢成分进行分离和定性、定量分析。结果CME容易被肠道菌群代谢，0.5h后样本中能检测出较高浓度代谢物；经LC-MS检测，主要的代谢物为木犀草素和芹菜素；随着代谢时间的延长，两者均出现降解代谢的现象，24h后几乎检测不到这两种代谢物；且人、大鼠和Beagle犬肠道菌群对CME的代谢较快，而家兔肠道菌群对CME的代谢过程比较平缓。结论在离体条件下，CME可以被人、大鼠、Beagle犬及家兔的肠道菌群代谢，主要的代谢物为木犀草素和芹菜素。

Objective To study and compare the metabolism of Chrysanthemum morifolium extracts(CME)in vitro intestinal flora from human, rat, Beagle dog, and rabbit. Methods Intestinal flora of human, rat, Beagle dog, and rabbit and CME were anaerobically cultured at 37℃. After being extracted by acetic ether, the main metabolites of CME were separated and quantified by HPLC. ResultsIn vitro, CME was easily metabolized by the intestinal flora and the main metabolites in incubation medium were determined in high concentration after 0. 5h. By LC-MS, luteolin and apigenin were identified, respectively;both metabolites were degraded with prolongation of incubation time and the concentrations of luteolin and apigenin were low after 24 h;moreover, CME was quickly metabolized by human, rat, Beagle dog intestinal flora, and gently by rabbit intesinal flora. ConclusionIn vitro, CME is easily metabolized by intestinal flora human, rat, Beagle dog, and rabbit and the main metabolites are luteolinand apigenin.

浙江省自然科学基金(Y204379);浙江省中药现代化专项资助(G20050066)