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[摘要]
目的研究茯苓原生质体制备及其再生条件。方法采用不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生条件进行研究。结果不同菌龄、酶液浓度、酶解时间、稳渗剂对茯苓原生质体产率及再生产生不同影响。结论Z10-2原生质体制备时菌丝最适菌龄为72 h,酶液浓度为3%,稳渗剂以0.5 mol/L的甘露醇为佳。Z10-3原生质体制备时菌丝最适菌龄为56 h,酶液浓度为3%,稳渗剂以0.6 mol/L的甘露醇为佳。Z10-2和Z10-3原生质体均以甘露醇作稳渗剂再生率较高,其最佳浓度为0.6 m o l/L。在该条件下Z10-2原生质体再生率为7.5×10-3,Z10-3为1.3×10-2。
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[Abstract]
Objective To study the conditions of separation and regeneration of Poria COC05 protoplast.Methods Separation and regeneration of P.COCOS protoplast under different ages of hyphae,enzyme consistency,and enzymolysic time and stabillizer.Results The results showed that the yield of the protoplast was different under different ages of hyphae,enzyme concentration,enzymolysic time,and stabilizer.Conclusion For strain Z10-2,its optimal age of hyphae,enzyme concentration,and stabilizer is 72 h,3%,and 05 mol/L mannitol,respectively.The strain Z10-3 is 56 h,3%,and 06 mol/L mannit01.The frequency of regeneration of the tWO strains is the best and 7.5 X10-3(strain Z10-2)and 1.3×10-2(strainZ10-2)separately when the stabilizer is 06 mol/I。mannit01.
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