目的为了选育出适合于江苏省栽培的优质丹参品种,也为丹参品种的系统选育提供一定的研究依据。方法采用HPLC法对不同类型丹参根内的脂溶性成分丹参酮ⅡA、隐丹参酮及水溶性成分丹参素、丹酚酸B进行了分析;采用紫外分光光度法分别对其总丹参酮和总丹酚酸的量进行分析,同时对4种类型丹参后期生物量进行了分析;并采用Exce l和SPSS软件对其生物量与脂溶性、水溶性成分间的相关性进行分析。结果4种类型的丹参的后期生物量积累不完全一致,并且总丹参酮、丹参酮ⅡA、隐丹参酮、丹酚酸B、丹参素和总丹酚酸量均存在一定的差异,生物量与有效成分间的相关性分析显示生物量与成分间呈负相关,脂溶性成分间存在较好的相关性,各类型的丹参酮ⅡA与隐丹参酮间的相关系数分别为0.943(ZY)、0.942(DY)、0.715(XY)、0.960(ZC);水溶性成分间也存在一定的相关性,各类型丹参的丹参素与丹酚酸B间的相关系数分别为0.766(ZY)、0.416(DY)、0.841(XY)、0.618(ZC)。但是两大类成分间不存在明显的相关性。各类型的水溶性成分与脂溶性成分的积累也存在一定差异。结论不同类型丹参中以小叶型为最佳,其脂溶性成分和水溶性成分量均高于其他3种类型,但其他类型的质量也远高于《中国药典》标准。

Objective To obtain excellent cultivars of Salvia miltiorrhiza cultivated in Jiangsu Province by selective breeding and provide some basic reference for systemic breeding.Methods Tanshinones(tanzhinoneⅡA and cryptotanshinone)and salvianolic acids(salvianolic acid A and B)in the roots of the four cuhivars of S。miltior,iza were determined bv HPLC.The total tanshinones and salvianolic acids were determined by spectrophotometry.Biomasses of four cultivars and correlative analysis between biomasses and effective components were analyzed with Excel and SPSS software.Results Accumulating of biomasses of the four cuhivars was coincident incompletely.Contents of total tanshinone,tanshinone ⅡA,cryptotanshinone,salvianolic acid B,danshensu,and total salviandic acids wer different.Correlative analysis showed that the relativity between biomasses and contents of effective components of four cultivars were negative correlative.Relativity among liposoluble components was distinct.Correlation coefficients between tanshinoneⅡA and cryptotanshinone of four cuhivars were 0.943(ZY),0.942(DY),0.715(XY),and 0.960(ZC),respectively.Correlation coefficients between salvialic acid A and B of fou cuhivars were 0.766(ZY),0.416(DY),0.841(XY),and 0.618(ZC),respectively.But the relativity between hydrosoluble and liposoluble components was not obvious.Accumulating trends of liposoluble and hydrosoluble components were different.Conclusion Contents of total diterpenoids and tanshinon I A in little lear cultivars are more than the contents of other three cultivars.The qualities of other three cuhivars are better than the qualities of S.miltior施iza recorded in Chinese Pharmacopoeia.

无锡市自然科学基金项目(CK20020001)