目的探讨萌动激活灵芝孢子对大鼠坐骨神经切断再吻合后受损伤运动神经元轴突再生的影响。方法对单侧坐骨神经切断再吻合后的大鼠ig给予萌动激活灵芝孢子,通过电生理、荧光金(FG)逆行标记和组织学等方法观察受损伤运动神经元轴突再生情况。结果坐骨神经切断再吻合6周后,灵芝孢子组坐骨神经再生轴突的动作电位潜伏期及峰峰值的恢复率以及运动神经元胞体的荧光金逆行标记率均明显高于对照组,灵芝孢子组大鼠腓肠肌萎缩程度也轻于对照组。结论萌动激活灵芝孢子能够促进大鼠坐骨神经切断再吻合后的脊髓受损伤运动神经元轴突再生。

Objective To explore the effects of germination.-activated Ganoderma spore(GASP)on axonal regeneration of injured motor neuron in rat spinal cord following sciatic nerve cut and anastomosis.Methods Rat was ig administered by GASP after the sciatic nerve was cut and anastomosed unilaterally and the axonal regeneration of injured motor neuron was observed by electrophysiology,Fluorogold(FG) retrograde labeling,and histologic techniques.Results After six weeks of sciatic nerve cut and anastomosis,the recovery ratio of latency and amplitude of action potential of regenerated axon at sciatic nerve and the ratio of FG-retrograde labeled motor neuronal soma in GASP group were all higher than those in control group.The gastrocnemius atrophy degree in GASP group was also lower than that in control group.Conclusion GASP can promote the axonal regeneration of injured motor neuron in rat spinal cord following sciatic nerve cut and anastomosis.