[关键词]
[摘要]
目的建立小鼠血清中芫花根总黄酮(TFRD)各成分的HPLC测定方法,探讨TFRD含药血清对正常小鼠细胞免疫功能的影响。方法HPLC法测定小鼠单次ig给药后不同时间点血药浓度;M TT法检测多次ig给药后含药血清对正常小鼠脾淋巴细胞增殖、NK与LAK细胞杀伤活性和腹腔巨噬细胞的吞噬功能等细胞免疫功能的影响。结果单次ig TFRD后血药浓度在20~30 m in达到高峰;TFRD含药血清能促进脾淋巴细胞增殖,增加NK和LAK细胞杀伤活性,增强腹腔巨噬细胞的吞噬能力。结论TFRD含药血清能增强正常小鼠细胞免疫功能。
[Key word]
[Abstract]
Objective To establish HPLC method for assaying total flavonoids from the roots of Daphne genkwa(TFRD)in serum of mice and to elucidate the effect of mice serum containing TFRD on cell immunity in mice.Methods TFRD Concentration in serum was determined from the mice received single ig TFRD at certain time intervals using HPLC method.The effects of TFRD serum on lymphocyte proliferation,killing activities of NK and LAK cell,and phagocytic activity of macrophage were detected bv MTT method.Results TFRD in serum reached its highest concentration in 20-30 min after ig administration.TFRD containing serum significantly improved the proliferation of lymphocyte,enhanced the killing activities of NK and LAK cells。and enforced the phagocytic activity of macrophage.Conclusion TFRD containing serum is an effective agents for enhancing cell immunity in mice.
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[基金项目]
教育部科学技术研究重点项目(03049);江苏省高校自然科学研究计划重点项目(02KJA360002)