[关键词]
[摘要]
目的对不同产地甘草Glycyrrhizauralensis的HPLC指纹图谱进行研究, 并对HPLC指纹图谱结果进行聚类分析, 以探讨产地对甘草药材活性成分积累的影响。方法以甘草药材所含活性成分为分析对象, 选择适宜的HPLC条件, 建立了甘草指纹图谱分析方法, 并对该方法进行了考察。以甘草酸、甘草苷、甘草素、异甘草苷、异甘草素5个活性成分峰面积的标准化结果对17批样品进行了聚类分析。结果方法学考察结果表明, 本研究建立的分析方法有较好的重现性, 不同产地甘草中甘草酸和甘草黄酮的量存在较大差别。结论甘草活性成分的积累与产地有一定的相关性。
[Key word]
[Abstract]
Objective To study HPLC fingerprints of Radix Glycyrrhizae from different habitats, develop the method of hierarchical cluster analysis by using the results of fingerprints, and discuss regional effect on accumulation of active ingredients of Radix Glycyrrhizae.Methods HPIC Fingerprint analysis method of active ingredients was developed and the method had been evaluated.Based on the standardized result of peak area of glycyrrhizin,liquiritin,liquiritigenin,isoliquiritin,and iS01iquiritigenin,seventeen batches of Radix Glycyrrhizae were classified and identified using hierarchical cluster analysis.Results The methodological evaluation showed that this method had a good repeatability.The amounts of glycyrrhizin and flavones in Radix Glycyrrhizae from various habitats are different.Conclusion Accumulation of active ingredients of Radix Glycyrrhizae has some relativity with habitats.
[中图分类号]
[基金项目]
国家“十五”科技攻关项目(2001BA701A41-1)