目的研究槲皮素对小鼠血吸虫病肝纤维化的治疗作用及其机制, 并与吡喹酮的作用进行对比。方法80只小鼠随机分为4组。其中3组小鼠于感染日本血吸虫8周后, 分别以槲皮素30mg/(kg·d)治疗8周、以吡喹酮500mg/(kg·d)治疗2d及不作任何治疗(模型组), 第4组小鼠作为正常组。应用HE染色法、RT-PCR及免疫组化染色, 观察和分析槲皮素及吡喹酮治疗前后各组小鼠肝组织病理改变及c-fos和c-junmRNA、转化生长因子β1(TGF-β1)和、型胶原表达的变化。结果槲皮素治疗后小鼠肝纤维化程度减轻, 与模型组相比, 肝组织中c-fos、c-junmRNA以及TGF-β1和、型胶原表达均明显降低(P<0.01);与吡喹酮组相比, c-junmRNA、和型胶原表达明显降低(P<0.05), 而c-fosmRNA和TGF-β1表达无显著改变。结论槲皮素对小鼠血吸虫病肝纤维化具有较好的治疗作用, 其远期抗肝纤维化效果优于吡喹酮。

Objective To study the therapeutical effects and mechanisms of quercetin on hepatic fibrosis in schistosome-infected mice and compare the effects with praziquantel.Methods Eighty mice were divided into four groups：among them three groups were infected by Schistosoma japonica.After 8 weeks,one group was treated with quercetin 30 mg/(kg·d)for 8 weeks,one group was treated with praziquantel 500 mg/(kg·d)for 2 d and the other was taken as model group without any treatment.The fourth group was taken as normal group.HE staining,RT-PCR,and immunohistochemlcal technique were used to observe the expression of hepatic c-los and c-jun mRNA, and the changes of hepatic TGF-β,type I,and type Ⅲ collagen in mice infected by S.japoniea before and after treatments.Results Quercetin obviously relieved the degree of hepatic fibrosis, significantly reduced the expression of c-fos,c-jun mRNA,TGFβl,type I, nd type collagen compared to the model group(P<0.01),and the expression of c-jun mRNA, type I,and typeⅢcollagen compared to the group treated with praziquantel(P<0.05),where as no difference in the level of c-fos mRNA and TGF-β1 between the two groups.Conclusion Quereetin has better therapeutieal effects on hepatic fibrosis in schistosome-infected mice and surpasses praziquantel in long term.