目的建立甘草药材的HPLC指纹图谱。方法采用反相高效液相色谱法,选用Zorbax SB-C₁₈柱(250mm×4.6mm,5μm)色谱柱;流动相为乙腈-1.2%醋酸溶液(梯度洗脱);分析时间70min;检测波长为254nm。结果建立了甘草药材的HPLC指纹图谱,标定了甘草药材38个共有指纹峰,方法学考察结果符合指纹图谱技术要求。结论方法稳定、可靠、重复性好,可为甘草药材质控标准的制定和含甘草的中药方剂的物质基础研究提供参考。

Objective To establish the HPLC fingerprints of Radix Glycyrrhizae. Methods The RP-HPLC method was used, Zorbax SB C₁₈ column (250 mm×4.6 mm, 5 μm) was employed; the acetonitrile-1.2% acetic acid (gradient elution) was used as mobile phase, analytic time was 70 min, and detective wavelength was at 254 nm. Results The HPLC fingerprints of Radix Glycyrrhizae were set up. The result showed that 38 peaks were common in different sources. The results of method validation met technical standard of fingerprints. Conclusion The method is stable and reliable with a good reproducibility and provides a reference standard for the quality control of Radix Glycyrrhizae.

国家自然科学基金重点资助项目(30030150)