目的研究水仙提取物对人类多发性骨髓瘤细胞系ARH-77的增殖及凋亡的影响。方法采用MTT法检测水仙提取物(1.25、2.5、5、10、20μg/mL)作用ARH-77细胞3d后细胞存活率的变化;采用荧光染色法观察水仙提取物(10μg/mL)作用3d对ARH-77细胞凋亡的影响;流式细胞仪分析细胞周期时相变化和检测细胞凋亡率;核仁组织区(NOR)相关嗜银蛋白(AgNOR)染色法检测细胞NOR的改变;透射电镜观察细胞亚微结构变化。结果水仙提取物能明显抑制ARH-77细胞增殖,降低细胞存活率(＜0.01),其IC₅₀为4.8μg/mL;促进肿瘤细胞凋亡(＜0.05),阻滞细胞周期于G₂/M期(＜0.01);降低AgNOR的数目(＜0.001)和促进AgNOR的融合(＜0.01);引起细胞亚微结构改变。结论水仙提取物能抑制ARH-77细胞增殖,诱导ARH-77细胞凋亡。

Objective To study the effects of extract from Narcissus tazetta var. chinensis on proliferation and apoptosis of human multiple myeloma cell line ARH-77. Methods The survival rate of cells were tested by MTT assay when cells were affected by 1.25, 2.5, 5, 10, 20 μg/mL of the extract for 3 d. After being induced by 10 μg/mL of the extract for 3 d, apoptotic cells were observed with fluorescence stain; changes of he csll cycle were analyzed by Flow Cytometry; the number and shape of nucleolar organizer region (NOR) were tested by AgNOR assay and submicroscopic structure were observed by transmission electron microscope. Results The extract from N. tazetta var. chinensis could significantly inhibit the proliferation and reduce the survival rate of ARH-77 cells (P0.01), the IC₅₀ was 4.8 μg/mL. Typical apoptosis morphologic cells were seen, the rate of apoptosis cells were increased (P0.05), and the cell cycle was arrested at G₂/M phase (P0.01) in the experimental group; then the extract from N. tazetta var. chinensis could make the number of AgNOR (P0.001) decrease, the cell fusion in AgNOR (P0.01) accelerate, and the submicroscopic structures of cell change. Conclusion The extract from N. tazetta var. chinensis could obviously inhibit the proliferation and induce the apoptosis of ARH-77 cells.

国家自然科学基金资助项目(39880021;30500269)