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[摘要]
目的采用加速溶剂提取法提取牛膝中的蜕皮甾酮,探讨其在中药质量控制中应用的可行性。方法以蜕皮甾酮为指标成分,采用RP-HPLC为检测方法,单因素考察法对影响加速溶剂提取牛膝中的蜕皮甾酮的因素进行考察。结果优选出了牛膝中蜕皮甾酮加速溶剂提取的最佳条件以无水甲醇为溶剂,药材的粒径为0.3~0.45mm,提取温度为100℃,提取压力10.34MPa,提取时间6min,提取1次。结论加速溶剂提取技术能够快速高效地提取牛膝中的蜕皮甾酮。
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[Abstract]
Objective To apply accelerated solvent extraction (ASE) technique to extract Achyranthes bidentata and to explore the application of this technique in quality control of Chinese materia medica. Methods Investigation of single factor was used to optimize the conditions that affected the efficiency for ASE from A. bidentata by RP-HPLC using ecdysterone as a quantitative marker. Results The optimized conditions for ASE of A. bidentata were obtained as follows: methanol as solvent, particle size between 0.3 and 0.45 mm, temperature at 100 ℃, pressure under 10.34 MPa, 6 min duration and once extraction. Conclusion ASE technique can be used to extract A. bidentata quickly and effectively.
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