目的建立赤、白首乌HPLC指纹图谱鉴定方法。方法用RP-HPLC(DAD)法,梯度洗脱,测定赤、白首乌、加工炮制品及其何首乌粉的指纹图谱,并作相似度比较分析。结果何首乌不同样品指纹图谱相似度较好,而何首乌与白首乌的指纹图谱具有明显区别,何首乌炮制前后、白首乌加工前后也有差异。结论HPLC指纹图谱具有重现性好、特征性强、方法简便等特点,可用于赤、白首乌药材的鉴别。

objective To establish HPLC fingerprint for the identification of Radix Polygoni MultL flsri and Radix C>nanchi AuHculati．Methods Chromatographic fingerprint of Radix Polygoni Multi-oH，Radix Cynanchi Auriculati，Radix Polygoni Multiflori Preparata，and“HEsHoU wU FEN”was determined by RP—HPLC(DAD)and the gradient elution mode applied in chromatographic separation，da—ta were analysed by Fingerprint Similarity Evaluation Software tO compare the similarity of samples．Re—suits Radix Polygoni Multiflori from different samples were of high similarity．but Radix Polygoni Mnz— and Radix Cynanchi Auriculati showed evident difference in fingerprint，and there was difference infingerprint through processing them．Conclusion HPLC fingerprint method is repeatable and feasible and can be used for the identification of Radix Po妇goni Multiflori and Radix Cynanchi AuriculatL

江苏省高校自然科学研究计划项目(03KJA360092)