目的研究姜黄素对小鼠腹水型肝癌高淋巴道转移细胞株(HCa-F)生长和转移行为的影响。方法体外实验中用15~250μmol/L姜黄素分别处理HCa-F细胞48h,以MTT法检测HCa-F细胞的生长活性;以生长曲线评估姜黄素对细胞增殖的影响;以流式细胞仪检测姜黄素对细胞周期分布的影响。体内实验中,于615小鼠腹腔、足垫接种HCa-F细胞,通过ip给药的方法,观察姜黄素对腹腔接种HCa-F细胞的615小鼠生存率的影响和对HCa-F细胞在615小鼠淋巴道转移的影响。结果姜黄素可抑制HCa-F细胞的生长,对HCa-F细胞生长抑制率为7.23%~82.23%,呈剂量依赖性,IC50为51.48μmol/L。以非细胞毒性最大剂量15μmol/L姜黄素作用48h后,HCa-F细胞的群体倍增时间增加,生长速度受到抑制,细胞周期被重新分布,阻滞于S期。姜黄素ip给药50mg/kg抑制小鼠腹水瘤的生长,延长荷瘤小鼠的生存期,使瘤细胞淋巴道转移率从70%下降到40%。结论姜黄素可抑制小鼠腹水型肝癌高淋巴道转移细胞株(HCa-F)的增殖与转移。

0bjective To observe the effect of curcumin on biological behavior such as proliferation and metastatic of ascitic hepatocarcinoma eell strain with high metastatic potential in 1ymphatic system of mice(HCa—F)．Methods In vitro，HCa—F cells were pretreated by curcumin at the concentration of 15～250,umol／L for 48 h，the growth of HCa—F cells was studied by MTT assay，the effect of eureumin on eell proliferation was observed by making growth curve．and the effeCt of curcumin on celI cycle distribution was evaluated by flow cytometry(FCM)．In vivo．HCa—F cells were inoculated to 615 mice via abdominalcavity and feet pads and the mice with HCa～F cells were given eurcumin(50 mg／kg．ip)．Effect of curcu—min on the formation of ascites，the life span of mice．and the rates of metastasis in lymphatic system ofmice were studied．Results The growth inhibition rates of curcumin on HCa—F reached 7．23％一82．23％in a dose—dependent manner and IC5。was 51．48卜mol／L．The growth speed of HCa—F cells treated by themaximum uneytotoxicity dose of cureumin 15 btmol／L for 48 h was inhibited，the multiplication time ofHCa，F cells was increased．and the eell cycle was redistributed with cells being arrested in S phase．Cur—cumin to tumor—bearing mice(50 mg／kg，ip)could inhibit the growth of ascitic hepatocarcinoma，prolongthe life span．and reduce the metastatic rate in lymphatic system from 70％tO 40％significantly．Conelu—sion Cureumin could inhibit the proliferation and metastasis of HCa—F cells of ascitic hepatocarcinoma in 1ymphatic system of mice．

国家自然科学基金资助项目(90209048);中国科学院重大方向性项目(KSCX2-SW-329,KGCX2-SW-213)