[关键词]
[摘要]
目的研究骨碎补超微饮片的HPLC指纹图谱,考察其有效成分,为骨碎补超微饮片的质控提供可靠方法。方法色谱柱为大连依利特公司Hypersil BDS C18(200mm×4.6mm,5μm);流动相为甲醇-0.1%磷酸水溶液梯度洗脱;柱温为25℃;体积流量为1.0mL/min;检测波长为283nm。结果初步建立了骨碎补超微饮片的HPLC指纹图谱,标定了8个共有峰。结论利用HPLC指纹图谱可以比较全面的控制骨碎补超微饮片的内在质量。
[Key word]
[Abstract]
objective To study Ultramicro Rhizoma Drynariae(herbal pieces prepared for decoction)and determine its constituents and content by HPLC fingerprint.Methods Chromatographic column:Hypersil BDS C18(200 mm×4.6 mm,5弘m);mobile phase:methanol一0.1%phosphoric acid in water in a elution;column temperature:25℃;flow rate:1.0 mL/min;wavelength:283 nm.ResultsThe fingerprint of Ultramicro Rhizoma Drynariae was established and eight common peaks were displayed in the fingerprint.Conclusion The Methods and data can be used to control the quality of Ultramicro Rhizoma Drynariae.
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[基金项目]
国家“十五”科技攻关项目(2001BA701A43);湖南省卫生厅中医药科研基金课题(24303);湖南省教育厅科研课题(04C452)