[关键词]
[摘要]
目的建立HPLC对白芍总苷胶囊中芍药内酯苷、芍药苷和苯甲酰芍药苷同时定量的分析方法。方法采用HPLC法。色谱柱Zorbax SB-C18色谱柱(250mm×4.6mm,5μm);流动相A为乙腈,B为0.015%磷酸溶液,梯度洗脱0min(8%A),5min(12%A),20min(20%A),25min(20%A);35min(45%A),40min(45%A);检测波长230nm;柱温30℃。结果芍药内酯苷、芍药苷和苯甲酰 药苷质量浓度与峰面积均呈良好的线性关系。平均回收率芍药内酯苷为96.99%,RSD为0.56%;芍药苷为103.6%,RSD为0.77%;苯甲酰芍药苷为104.2%,RSD为1.35%(n=5)。结论该方法分离度好,简便易行,具有良好的重现性,可用于白芍总苷胶囊中芍药内酯苷、芍药苷和苯甲酰芍药苷的同时测定。
[Key word]
[Abstract]
0bjective An HPLC method was developed for the determination of albiflorin, benzoylpaeono“n in Total Glucoside of Paeony Capsule(TGPC).Methods HPLC analysis was performed on a Zorbax SB—C18 column(250 mm×4.6 mm,5川n)with mixture of acetonitrile(A)and 0.015%phosphoric acid solution as mobile phase in gradient mode.The concentrations of solventA were 8%,12%,20%,20%,45%,and 45%at 0,5,20,25,35,and 40 rain,respectivelyt the detection wavelength was 230 nm and the column temperature WaS 30 C.Results The standard curves of albiflorin,paeoniflorin,and benzoylpaeoninorin showed a good linearity and the average recoveries were96.99%with RSD 0.56%for albiflorin,103.6%with RSD 0.77%for paeoniflorin,and 104.2%with RSD 1.35%for benzoylpaeoniflorin o一5),respectively.Conclusion The method is quick,simple,and repeatable for the determination of albiflorin.paeoniflorin. and benzoylpaeoniflorin in TGPC simultaneously.
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[基金项目]
国家科技部资助项目(2002BA906A29);国家中医药管理局资助项目(2004ZX01)