2b)主要成分的相对分子质量,为建立对照品的标准提供依据?方法PSM2b经Sepharose 4B柱分离,冷冻干燥后得到丹皮多糖的两个成分PSM2b-A和PSM2b-B,采用HPLC法检测均一性和相对分子质量;并分别以0.7%Na2SO4溶液和超纯水作为流动相进行比较?结果PSM2b-A为均一性的糖蛋白,是PSM2b的主要成分?流动相为0.7%Na2SO4溶液时,其相对分子质量为3.596 2×105,分散度指数(D)为4.15?流动相为超纯水时,其相对分子质量为4.758 5×105,分散度指数为1.03?结论采用HPLC法测定丹皮多糖主要成分的相对分子质量时,不同流动相测定的结果有差异,流动相采用超纯水比0.7%Na2SO4溶液更为适合? height=38 width=245 style="color:#000000;font-size:9.0pt;font-weight:400;font-style:normal;text-decoration:none;text-align:general;vertical-align:middle;white-space:normal;mso-protection:locked visible;height:28.50pt;width:183.75pt;">目的测定丹皮多糖2b(PSM_2b)主要成分的相对分子质量,为建立对照品的标准提供依据?方法PSM_2b经Sepharose 4B柱分离,冷冻干燥后得到丹皮多糖的两个成分PSM_2b-A和PSM_2b-B,采用HPLC法检测均一性和相对分子质量;并分别以0.7%Na₂SO₄溶液和超纯水作为流动相进行比较?结果PSM_2b-A为均一性的糖蛋白,是PSM_2b的主要成分?流动相为0.7%Na₂SO₄溶液时,其相对分子质量为3.596 2×105,分散度指数(D)为4.15?流动相为超纯水时,其相对分子质量为4.758 5×105,分散度指数为1.03?结论采用HPLC法测定丹皮多糖主要成分的相对分子质量时,不同流动相测定的结果有差异,流动相采用超纯水比0.7%Na₂SO₄溶液更为适合?

Objective To establish a sensitive HPIC method with UV detection for the determination of tanshinoneⅡ_A（.I'SⅡ_A）and crypfotanshlnone（CT）in rat plasma and to study pharmacokineties of Radix Salviae Ztiorrhizae（RSM）liposoluble components in rat in vivo.Methods TSⅡ_A and CT in plasma of rat at different sampling time were determined by pretreatment with Gemifibrozil as internal standard,the protein was precipitated in methanol solution after iv administration of alcohol extract of RSM 5 rag/per animaI.The irnean plasma concentration time curve was protracted and pharmacokinetic parameters were calculated by 3p87.Results The main pharmacokinetie parameters of TSⅡ_A were as follows:l/nwas（O.088±0.024）h;was（2.1士0.7）h;Vwas（0.8±0.6）I;CI was（2.0士1.1）I/h;AUCo 4 and AUCo-were（2.2±O.9）mg·h/I and（3,4±1.7）mg·h/f,respectively.Conclusion The concentration-time curve of TSⅡ_A can befitted to two-compartment model after ethanol extract of RSM iv administrated.Concentration of TSⅡ_A in rat plasma declines rapidly with large apparent volume Of distribution.CT decreases also rapidly and it is difficult to acquire its pharmacokinetic parameters in rat.

国家自然科学基金资助项目(3007093530271614)