目的比较江苏道地药材邳州银杏叶在不同采收期的指纹图谱。方法采用HPLC-DAD方法。色谱条件为:色谱柱:AlltimaC18柱(250mm×4.6mm,5μm);流动相:乙腈(A)-0.5%磷酸水(B)进行梯度洗脱,15%～27%A(0～40min),27%～15%A(40～60min),15%A(60～75min);体积流量:1mL/min;柱温:20℃;检测波长:360nm。并应用计算机辅助相似性评价系统对银杏叶指纹图谱进行了相似度分析。结果同一树龄不同采收期的银杏叶中,二至四年生银杏叶分别在不同采收期指纹图谱相似度较高(>0.90);五年生和六年生银杏叶分别在不同采收期指纹图谱相似度变化范围较大(0.86～0.97)。同一采收期不同树龄的银杏叶中,5～7月不同树龄银杏叶指纹图谱相似度较高(>0.90);8～10月不同树龄银杏叶指纹图谱相似度变化范围较大(0.84～0.99)。结论本研究为控制邳州银杏叶内在质量建立了指纹图谱方法,为银杏叶的合理采收提供了科学依据。

Objective To compare the fingerprints of Pizhou Ginkgo biloba leaves at different trees ages in different collecting seasons. Methods HPLC-DAD was applied. The conditions were as follows: Alltima C_ 18 column (250 nm×4.6 mm, 5 μm); mobile phase: acetonitrile (A)-0.5% phosphoric acid solution (B) (gradient elution), 15%-27% A (0-40 min), 27%-15% A (40-60 min), 15% A (60-75 min); flow rate: 1 mL/min; colum temperature: 20 ℃; detecting wavelength: 360 nm. Computer Aided Similarity Evaluation System was used to analyze the similarity of G. biloba leaves. Results Among the same-age but different collecting-time, the similarities of fingerprints of G. biloba leaves from 2 to 4 aged trees are relatively higher (0.90); but those from 5 to 6 aged trees change from 0.86 to 0.97. Among the different-age but same collecting-time, the similarities of fingerprints of G. biloba leaves collected from May to July were relatively higher (0.90); but those from August to October changed from 0.84 to 0.99. Conclusion The research has established a method of using fingerprint to control the internal quality of Pizhou G. biloba leaves and has provided a scientific basis for the reasonable collection of G. biloba leaves.

国家科技部“中药现代化研究与产业化开发”资助项目(99-929-01-01AE99206)