目的建立粉葛的组织培养体系;培育粉葛同源四倍体新种质.方法以MS培养基为基本培养基,培养外植体茎尖或茎段获得组培再生植株;用不同体积分数的秋水仙素对无菌苗茎尖进行不同时间的处理,诱导遗传物质加倍的同源四倍体.结果其最适愈伤组织诱导培养基为MS+6-BA 1.0mg/L+2,4-D 1.0mg/L+ NAA 0.2mg/L,分化和增殖培养基为MS+6-BA 1.0mg/L+2,4-D 0.2mg/L,生根培养基为1/2 MS+IBA 0.2mg/L;从长出约1cm的丛生芽上切取0.3~0.5cm的茎尖用0.4%~ 0.5% 的秋水仙素处理48h效果较好,体积分数过低或过高和处理时间太短或太长都不易成功.结论同源四倍体粉葛为葛根中药用物质质量分数的提高提供了基础.

Objective To establish a tissue culture system ofPueraria thomsonii and to cultivate a new breed autotetraploid.Meth-ods Using the MS medium as the basic medium,the explants shoot apexes and stem segments were cultured tO differentiate the regene-rations.The autotetraloid with genetic material doubled was achieved by treating shoot tips with colchicine of different concentratio-ns for different long times.Results The proper media MS+6-BA 1.0mg/L+2,4-D 1.0mg/L+NAA 0.2mg/L is for protocorn inducing,MS+6-BA 1.0mg/L +2,4-D 0.2mg/L for diffrentiation and propagation,and 1/2 MS+IBA 0.2mg/L for rooting.The best effect of the autotetraploid induction can be achieved by treating the 0.3-0.5cm shoot tips cut from the 1cm plantlets with 0.4%-0.5%colchicine for 48h,and it is unlikely to succeed by treating shoot tips with colchicine of very low or high concentration or for very long or short time.Conclu-sion The autotetraploid gives a chance to improve the content of medical materials in the root ofP.thomsonii.