目的研究蝎皮蛋白质组分对正常小鼠免疫功能的影响,为蝎皮开发利用和发现新的活性物质提供实验依据.方法采用刃天青和MTT法检测蝎皮水溶性总蛋白(STP)、总角蛋白(STKP)及水溶性蛋白组分Ⅱ(STPⅡ)对正常小鼠T、B淋巴细胞转化、NK细胞杀伤活性的作用.结果STP和STKP ig给药后,小鼠脾T淋巴细胞增殖反应高于正常对照组,但只有STP小剂量组与对照组比较差异显著(P<0.001).B淋巴细胞增殖反应结果显示STKP大、小剂量均可加强增殖反应,与对照组比较差异极显著(P<0.05、0.01).STP和STKP大、小剂量组NK细胞杀伤活性与对照组相比有升高趋势.STPⅡ两个剂量组100、200 mg/kg ig给药均能明显抑制正常小鼠T、B淋巴细胞增殖反应,与对照组相比差异极显著.STPⅡ大剂量组NK细胞杀伤活性与对照组相比有降低趋势,但无统计学意义.结论STP和STKP具有-定的提高免疫功能的作用,STPⅡ则具有抑制免疫功能的活性.揭示蝎皮蛋白质组分具有免疫调节药理活性,值得深入研究.

Objective To study effects of scorpion tegument protein(STP)on immunological function in normal mice and provide experimental data for searching new compounds.Methods Resazurin and MTT assay were used to detect the effects of scorpion tegument water-soluble protein,scorpion tegument keratin protein(STKP)and STP componentⅡ(STPⅡ)on the transformation of T and B lymphocytes,and NK cell activity in normal mice.Results By ig STP and STKP,the proliferation response of T lymphocyte in murine spleen was higher than that in normal control group,while only there was significant difference between low-dose of STP group compared with the normal control group (P<0.001).Administration of STKP by ig in both large-·and small--dose groups also significantly increased B lymphocytes proliferation response(P<0.05,0.01).Activity of NK cells had raising trend in high-and low-dose of STP and STKP groups compared with the normal control group.However,STPⅡ(100 and 200 mg/kg)showed extremely inhibitory effects on T and B lymph-ocyte proliferation response.STPⅡ(200 mg/kg)could inhibit activity of NK cell,but there was no significant difference compared with the control group. Conclusion STP and STKP possess potential immuno-improving effects and STP II has immuno-suppressive effects.It indicates that STP has the immuno-regulating activity that should be studied furtherly.

国家自然科学基金资助项目(30171148,3001161940);国家中医药管理局中医药科技研究专项(02-03ZP08)