目的观察雷公藤甲素对胶原诱导的关节炎大鼠关节局部热休克蛋白(HSPs)和主要组织相容性复合体Ⅰ类分子(MHC-Ⅰ)表达的影响.方法建立胶原诱导的关节炎大鼠模型,雷公藤甲素按40μg/kg im给药,运用逆转录聚合酶链反应(RT-PCR)和免疫酶组织化学染色的方法,观察大鼠关节滑膜细胞和软骨细胞HSP60、 HSP70和MHC-Ⅰ类分子的表达情况.结果与正常对照组相比,模型组大鼠关节滑膜细胞及软骨细胞HSP60、HSP70和MHC-Ⅰ类分子的表达均显著增高(P <0.05),与模型组相比,雷公藤甲素可以下调关节炎大鼠关节局部HSP60、HSP70和MHC-Ⅰ类分子的表达(P<0.05).结论降低关节炎大鼠关节局部软骨细胞和滑膜细胞异常表达的HSPs与MHC-Ⅰ类分子的表达,可能是雷公藤甲素治疗类风湿关节炎(RA)的作用机制之-.

Objective To investigate the effects of triptolide on the expression of heat shock proteins(HSPs)and major histocompatibility-I(MHC-I)molecule in synoviocytes and chondrocytes of collagen-induced arthritis rats.Methods The experimental Wistar rat model of collagen-induced arthritis was established.The rats in triptolide group were administered with triptolide at 40μg/kg by im.The exp-ression of HSP60,HSP70,and MHC-I molecule in synoviocytes and chondrocytes was detected by RT-PCR and immunohistochemical staining. Results To compare with normal group.the expression of HSPs and MHC-I molecule increased remarkably in synoviocytes and chondroc-ytes of model rats(P<0.05).Triptolide could reduce the expression of these molecules in inflammatory joints rats(P<0.05) Conclusion Triptolide may play its curative role via down-regulating the exprssion of HSPs and MHC-I molecule in synoviocytes and chondrocytes of rheumatoid arthritis(RA).

国家自然科学基金资助项目(30070961)