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[摘要]
目的建立雷公藤及其片剂中雷公藤红素的HPLC测定方法.方法HPLC外标法.Agilent Zorbax SC-C18柱为色谱柱,甲醇-1%醋酸溶(87:13)为流动相,检测波长为425 nm,体积流量为1.0 mL/m in.结果雷公藤红素线性范围为40.96~204.8μg/mL(r=0.999 6),药材的平均回收率为98.37%,RSD为1.01%(n=9),制剂的平均回收率为98.59%,RSD为1.18%(n=9).结论本方法操作简便、准确,可作为雷公藤类药物中雷公藤红素的定量分析方法.
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[Abstract]
Objective To determine the content of tripterine inTripterygium wilfordii and its tablets by HPLC.Methods An ext-ernal standard method by HPLC with Zorbax C18 column as fixed phase and methanol-1%HAc(87:13)as mobile phase was adopted. The detection wavelength was 425 nm and the flow rate was 1.0 mL/min.Results The linear range for tripterine was 40.96~204.8 μg/mL(r-0.999 6).The average recovery of Chinese medicinal materials was 98.37%and RSD was 1.01%(n=9);the average recovery of prepara-tion sample was 98.59%and RSD was 1.18%(n=9).Conclusion The method is simple and accurate,which can be adoptable for quantitat-ive analysis of tripterine in the plants of Tripterygium L.
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[基金项目]
教育部优秀青年教师资助计划资助项目