目的 研究奇任醇的抗炎作用及对免疫系统的影响。方法 建立大鼠佐剂性关节炎模型,观察奇任醇对大鼠足肿胀、脾淋巴细胞增殖、T淋巴细胞亚群的影响;观察奇任醇对小鼠耳廓肿胀、大鼠肉芽肿的抗炎作用;通过碳粒廓清、小鼠迟发型变态反应和血清溶血素水平实验,评价奇任醇对小鼠非特异性和特异性免疫功能的影响。结果 奇任醇能够抑制佐剂性关节炎大鼠的足肿胀,增强佐剂性关节炎大鼠脾淋巴细胞增殖能力,调节T淋巴细胞亚群比例;抑制小鼠耳廓肿胀和大鼠肉芽肿增生;抑制正常小鼠特异性免疫功能。结论 奇任醇可抑制佐剂性关节炎大鼠的原发性与继发性足肿胀,抑制急、慢性炎症反应,调节机体免疫功能。

Objective To study the anti-inflammatory and immuno-regulating effect of kirenol. (Methods) Adjuvant arthritis rat model was made by means of Freunds complete adjuvant, then the changes of paw swelling, proliferation of splenic lymphocyte and T lymphocyte subpopulation ratio of adjuvant arthritis rats were observed. The anti-inflammatory effect of kirenol was evaluated using ear edema model induced by xylene and granuloma model induced by cotton balls, and further the effect of kirenol on specific and non-specific immune response was evaluated by delayed hypersensitivity of murine auricle swelling and serum hemolysin level test and carbon clearance rate in mice. Results Kirenol can inhibit paw swelling, boost up the proliferation of splenic lymphocyte of adjuvant arthritis rats, regulate T lymphocyte subpopulation ratio, and also can inhibit ear edema in mice, granuloma weight in rats, and specific immune response of normal mice.Conclusion Kirenol can control the primary and secondary paw swelling of adjuvant arthritis rats, inhibit acute and chronic inflammation, and regulate immunologic function.

北京市科委基金资助项目(京科条发[2001]538号)