目的 观察川芎嗪(TMP)对嘌呤2 X(P2 X)受体激动剂[三磷酸腺苷(ATP)和α,β-亚甲三磷酸腺苷(α,β- me ATP) ]、前列腺素E2 (PGE2 )及P物质(SP)所致大鼠足底急性伤害性反应的影响。方法 通过大鼠痛行为反应确定局部应用TMP对ATP等P2 X受体激动剂、PGE2 及SP所致大鼠足底急性伤害性反应和足底炎症水肿的影响。结果 TMP (10 mm ol/L )明显抑制ATP (1μm ol/L )或α,β- m e ATP (0 .6μm ol/L )引起的大鼠足底急性伤害性反应。TMP(10 m mol/L)可抑制PGE2 (5 μmol/L)或α,β- me ATP(0 .2 μm ol/L)加PGE2 (5 μmol/L )引起的伤害性反应。TMP (10 m mol/L )不影响α,β- me ATP (0 .2μmol/L )加SP (10μmol/L )引起的伤害性反应。TMP对PGE2 、SP或α,β- m e ATP分别加PGE2 或SP引起的大鼠足底炎症水肿无明显影响。结论 TMP主要通过抑制P2 X受体兴奋介导的伤害性信息传递产生抗伤害性反应作用。

Objective To observe the effects of tetramethylpyrazine(TMP)on acute nociception in rat hindpaw induced by purine 2X(P2X)receptor agonists，such as adenosine triphosphate(ATP)and d，pmeATP，prostaglandin E₂(PGE₂)，and substance P(SP)．Methods The effects of TMP administered intraplantarlly on the acute nociception induced by P2X receptor agonists，PGE₂，or SP in the rat hindpaw were investigated by the method of the behavioral study．Resuits TMP(10μmol/L)significantly depressed the acute nociception induced by ATP(1/μmol/L)or a，β-meATP(O．6/μmol/L)in the rat hindpaw．TMP(10 μmol/L)could inhibit the acute nociception induced by PGE₂(5 gmol/L)or a，t3-meATP (O．2/μmol/L)coinjected with PGE₂(5/1mol/L)．TMP(10 μmol/L)could not affect the acute nociception induced by a，β-meATP(O．2μmol/L)coinjected with SP(1 0/μmol/L)．TMP could not obviously affect the inflammatory edema in rat hindpaw induced by the local administration of PGE₂，SP，or a，13一meATP coinjected with PGE 2 or SP individually．Conclusion The antinociceptive effects of TMP may mainly be associated with inhibiting the transmission of nociceptive information mediated by P2X receptor activation．

国家自然科学基金项目(30260030)