目的 观察四物汤及其化学成分组合对人骨髓基质细胞系HFCL细胞增殖及造血相关基因表达谱的影响,探讨其促HFCL 细胞增殖的可能机制。方法 MTT法测定四物汤化学成分组合对体外HFCL 细胞增殖的影响;运用流式细胞术分析细胞周期的变化;制备人造血相关细胞因子寡核苷酸芯片并检测四物汤及其化学成分组合所致的造血相关细胞因子基因差异表达情况。结果 四物汤化学成分组合增强HFCL细胞的能量代谢,促进HFCL 细胞增殖;四物汤化学成分组合组处于G0 / G1 期的细胞显著少于空白组;增殖指数(PI)则显著大于空白组;IL2 - Rα、NF-κB、TPO基因表达上调。结论 四物汤化学成分组合可通过上调IL2 - Rα、NF-κB、TPO基因表达而促进HFCL 细胞增殖。

Objective To investigate the effects of component combination in Siwu Decoction (CCSWD)on the proliferation of human bone marrow stromal cell lines HFCL and hematopoiesis—related gene expression and to probe into the meehanism of the stimulative effects of SWD and CCSWD on the pro—liferation of HFCL cells．Methods MTT and FCM were used for assaying the proliferation potency and cell cycle of HFCL cells，respectively．A human hematopoiesis—related cytokine oligonuleotide microarray was prepared to investigate the diversity of gene expression of HFCL cells in SWD and CCSWD groups．Results The energy metabolism and proliferation potency of HFCL cells treated with CCSWD were more prompted than that of the control group．Meanwhile，the percentage of HFCL cells in G₀/C₁ phase treated with CCSWD was significantly lower，while the proliferation index(PI)was higher than that of the control group．Compared to the control group，expressions of IL₂一Ra，NF—KB，and TPO were up—regulated in CCSWD group．Conclusion The proliferation of HFCL cells could be facilitated by CCSWD with up—regu—lating the expressions of IL，一Ra，NF—IcB，and TPO．

国家自然科学基金项目(30271617;30070913);“十五”军队重点项目(01Z019);北京市“二四八”重大创新工程项目(H010210220113)