目的建立小鼠口服赤芍胡椒复方后血浆中芍药苷浓度的RP-HPLC定量分析方法.方法以甲醇-水(38:62)为流动相.色谱柱为Kromasil C18(250mm×4.6mm,7μm),体积流量为0.5mL/min,以二极管阵列检测器获取230nm波长下数据,采用外标定量法.结果芍药苷与血浆中其他成分能很好分离,芍药苷在5.0～250.0ng/μL线性关系良好(τ=0.9999),最低检测质量浓度为1.49ng/μL,平均回收率大于90%.结论本方法准确性、灵敏度、专一性高,可为芍药苷的体内血浆药物浓度分析提供有意义的方法学参考.

Objective An HPLC method was established for determination of paeoniflorin in plasma after ig compound decoction of Radix Paeoniae Rubra with Fructus Piperis to mice．Methods The conditions of chromatography：Kromasil C18 column(250mm×4．6 mm，7 pm)was used with a mobile phase of CH30H—H20(38：62)；flow rate was 0．5 mL/min；the detecting wavelength was 230 nm；exterHal standard method was quantitative analysis method．Results Paeoniflorin was fully separated ftom the other components in plasma．The 1inear range was 5．O一250．0 ng/μL(r一0．999 9)，the lowest detectability was 1．49 ng/μL，the average recovery was higher than 90％．Conclusion This method specially provides an accurate and sensitive way in detecting the in vivo blood concentration of paeoniflorin in plasma.

国家中医药管理局科研基金资助项目(02-03ZP50);四川省中医药管理局科研基金资助项目(200217)