[关键词]
[摘要]
目的研究龙葵碱对H22荷瘤小鼠肿瘤细胞膜唾液酸(SA)水平和封闭度的影响。方法建立H22荷瘤小鼠模型,荷瘤小鼠分别sc生理盐水、环磷酰胺、龙葵碱(9.375、18.75、37.5mg/kg)。采用比色法测定H22小鼠肿瘤细胞膜SA水平和封闭度。结果龙葵碱可剂量依赖性地降低荷瘤小鼠肿瘤细胞膜SA水平和肿瘤细胞膜封闭度。结论龙葵碱通过降低H22荷瘤小鼠肿瘤细胞膜SA水平和肿瘤细胞膜封闭度起抗肿瘤作用。
[Key word]
[Abstract]
Objective To study the effects of total alkaloid from Solanum nigrum (TASN) on sialic acid (SA) and blocking degree of tumor cell membrane in H_(22) tumor-bearing mice. Methods H_(22) tumor-bearing mice model was established and H_(22) tumor-bearing mice were given NS, Cytoxan (CTX), and TASN (9.375, 18.75, and 37.5 mg/kg) by sc, respectively. SA content and blocking degree of tumor cell membrane in H_(22) mice were determined by chromatometry. Results TASN could decrease the SA content and the blocking degree of tumor cell membrane in H_(22) tumor-bearing mice in a dose-dependent manner. Conclusion TASN shows the antitumor activity through decreasing the SA content and blocking degree of tumor cell membrane in H_(22) tumor-bearing mice.
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[基金项目]
国家自然科学基金资助项目(30400591);黑龙江省自然科学基金资助项目(D2004-13);哈尔滨市青年科学基金资助项目(2004AFQXJ035)