[关键词]
[摘要]
目的对陕西产秦艽Gentianamacrophylla的化学成分进行系统研究。方法利用硅胶柱及其他多种色谱手段对秦艽的化学成分进行分离;用UV、IR、MS、1H-NMR、13C-NMR等技术及与对照品对照进行鉴定。结果从脂溶部位得到5个化合物,分别鉴定为:N-正二十五烷基-2-羧基苯甲酰胺(N-pentacosyl-2-carboxy-benzoylamide,)、5-羧基-3,4-二氢-1H-2-苯并吡喃-1-酮(5-carboxyl-3,4-dihydrogen-1H-2-benzopyran-1-one,)、红百金花内酯(erythrocentaurin,)、栎瘿酸(roburicacid,),齐墩果酸(oleanolicacid,)。结论、为两个新化合物,分别命名为秦艽酰胺和红百金花酸,为首次从本属植物中分得。
[Key word]
[Abstract]
Objective To systematically study the chemical constituents in the roots of Gentiana macrophylla from Shaanxi Province. Methods Various column chromatographic techniques were used for isolation and purification of the principles. The structures were elucidated on the basis of spectral data (UV, IR, MS, (~1H-NMR), (~(13)C-NMR), (~1H-~1H COSY) and (~1H-~(13)C COSY), HMBC, and HMQC) and identified by comparing with the authentic substance. Results Five compounds were got from fat-soluble fraction. They are identified as: N-pentacosyl-2-carboxy-benzoyl amide (Ⅰ), 5-carboxyl-3, 4-dihydrogen-1H-2-benzopyran-1-one (Ⅱ), erythrocentaurin (Ⅲ), roburic acid (Ⅳ), oleanolic acid (Ⅴ). Conclusion Compounds Ⅰ and Ⅱ are two novel ones. They are named as qinjiaoamide and erythrocentauric acid, respectively. Compound Ⅲ is isolated from the plants of Gentiana L. for the first time.
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[基金项目]
国家科技部“十五”攻关计划“中药现代研究与产业人开发”项目资助(2001BA701A07-07)