目的 研究不同浓度的稀土元素镧(La³⁺)对3种大黄根生物量和蒽醌次生代谢产物产量的影响.方法采用统计学分析方法，研究稀土元素La³⁺对两种掌叶大黄单克隆毛状根DH5c、DH7a和液体培养非转化根NOR生物量和蒽醌化合物合成的影响.结果 La³⁺浓度对大黄根生物量积累有显著影响，培养基中La³⁺为10 mg/L时，对大黄根生长表现出明显的抑制作用;La³⁺浓度对3种大黄根的蒽醌产量有极显著影响，当培养基中La³⁺1.0mg/L时，蒽醌产量最高;经稀土离子处理的3种大黄根中芦荟大黄素和大黄酸显著高于大黄素、大黄酚和大黄素甲醚.结论 培养基中添加适宜浓度的稀土离子La³⁺对掌叶大黄单克隆毛状根的生物量积累和蒽醌类化合物的合成具有明显的促进作用.

Object To study the effect of rare-earth elements La³⁺ on growth of hairy roots mal of Rheum palmatum L and their yield of anthraquinones.Methods The biomass and anthraquinone yield of two hairy clones DH5c，DH7a，and normal root NOR cultures of R.palmatum were evaluated statistically after various concentration of La³⁺were administrated into culture medium.Results Significant differences of biomass yield and anthraquinone yield were shown among six La³⁺ concentrations，in which 10 mg/L showed obvious inhibitory effect for root growth and 1.0 mg/L was the best for anthraquinone yield.In general，aloe-emodin and rhein were predominant in five anthraquinones after treated with the rare-earth element.Conclusion The growth of hairy root cultures of R.palmatum and their anthraquinone yield are greatly promoted by addition of La³⁺ to the medium.