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[摘要]
目的 对不同植物激素及其配比对黄花乌头体细胞胚胎诱导及植株再生进行研究,为药用植物黄花乌头的资源开发提供理论依据。方法 以黄花乌头的茎、叶、胚轴、胚根和子叶为外植体考察不同植物激素对黄花乌头愈伤组织诱导、体细胞胚胎发生及植株再生的影响。结果 外植体接种于MS +2,4 D 1mg/L +6 BA 0 5 mg/L的培养基上愈伤组织诱导率最高,愈伤组织在MS +2,4 D 0 2mg/L +6 BA 0 5mg/L的培养基上连续继代几次后,分化出淡绿色具有圆锥状或原球状突起的胚性愈伤组织。胚性愈伤组织能够在MS+6 BA 2mg/L的培养基上形成不定芽,该芽能够在MS +IBA 0 5mg/L的培养基上生根,形成完整的再生植株。结论 利用体细胞胚胎发生技术可以成功地得到再生植株,从而为濒危植物黄花乌头的资源开发和保护提供新途径
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[Abstract]
Object To develop new resources of medicinal plan t of Aconitum coreanum (Lévl.) Rapaics by the method of plant cel l biotechnology. Methods The segments of stems, leaves, embr yonal axises, radicles, and cotyledon of A. coreanum were used a s the explants. The MS medium supplied with different auxin and cytokinin was e xamined. Results The calli were induced from explants on the MS medium supplied with 2, 4-D 1 mg/L and 6-BA 0.5 mg/L in the period of 30 d. And the calli were differentiated into green conus or globe embryo after se veral subcultures period on medium MS supplied with 2, 4-D 0.2 mg/L and 6-BA 0.5 mg/L. The adventitious buds differentiated could be developed from the so matic embryo callus into roots on medium MS supplied with IBA 0.5 mg/L, and the n the intact plantlets were obtained. Conclusion The plantle ts of A. coreanum can be obtained by the techniques of somatic embryogenesis and it is indicated that this may be a new way in developing new r esource and protecting of A. coreanum.
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