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[摘要]
目的 对野生菱和栽培菱种rDNAITS片段进行分析,探讨该片段在两大群体中的系统学及鉴别研究意义。方法 对菱的rDNAITS区进行了PCR扩增、测序,运用Clustal、Mega2.0等软件对ITS区进行序列分析鉴别。结果 获得rDNAITS1、ITS2和 5.8SrDNA完整序列,10个居群菱的ITS1与ITS2序列的长度分别为 2.34~2.36bp和 2.2 0~ 2.2 1bp,5.8S区均为 16.4bp,不同居群的碱基差异为 0.2 2%~ 2.94%。变异位点数为 16个,信息位点数 6个。用NJ法根据ITS序列数据构建系统发生树。结论 尽管菱属各居群间rDNAITS的差异百分率较小,其亲缘关系较近,但根据ITS序列的特征可以很好地鉴别野生菱、栽培菱,菱属植物有可能都起源于同一种野生类居群。
[Key word]
[Abstract]
Object To analyze the rDNA ITS sequences between wi ld plants and cultivars of Trapa L. and study the utility in p hylogenesis and identification of these two groups. Methods The ITS gene fragments were PCR amplified and sequenced. The rDNA ITS regions w ere analyzed by means of the software of Clustal and Mega 2.0. Results The rDNA sequences of 234-236 bp ITS1, 220-221 bp ITS2 gene fragment, and 5.8 S rDNA for 164 bp evenly were obtained from ten populations of Trapa L. The intraspecific substitution varies from 0.22% to 2. 94%. The variable sites are 16 while informative sites are six. The phylogenet ic tree based on ITS data was set up by NJ method. Conclusion ITS sequence is a pretty good molecular marker which can identify wild plants of Trapa L. from their cultivars. Diversity of ITS in differen t populations is less at intraspecific level. It is infered that the plants of Trapa L. may be derived from the same population of one species .
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[基金项目]
江苏省教育厅自然基金资助项目 ( 0 1KJB180 0 0 5 )