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[摘要]
目的 研究芦荟多糖对S180 荷瘤小鼠红细胞膜功能的影响。方法 采用荧光分光光度法、SDS 聚丙烯酰胺凝胶电泳以及唾液酸试剂盒分别测定S180 小鼠红细胞膜脂流动性、膜交联蛋白、带 3蛋白以及膜唾液酸 (SA)含量。结果 两种芦荟多糖各剂量组均能不同程度地提高S180 荷瘤小鼠降低的红细胞膜脂流动性、带 3蛋白和SA的含量,降低红细胞膜交联蛋白的含量,且中剂量均非常显著 (P <0.0 1)。结论 芦荟多糖对S180 荷瘤小鼠红细胞膜功能具有促进作用,这可能是芦荟多糖抗肿瘤作用的主要机制之一。
[Key word]
[Abstract]
Object To study the effect of aloe polysaccharide (AP) on erythrocyte membrane function of S180 mice. Methods Lipid fluidity, the cross2linking protein content, Band 3 protein and sialic acid (SA) con-tent of erythrocyte membrane in S180 mice were measured by spect rofluorimetry, SDS2PAGE, and SA reagent test Kit, respectively. Results Different dosage groups of the two kinds of AP raised the lipid fluidity of ery-throcyte membrane, increased Band 3 protein and SA content s of erythrocyte membrane, while decreased the cross-linking protein content at different level. The effect of middle dosage groups of AP were very remarkable( P < 0101) . Conclusion AP can improve the erythrocyte membrane function of S180 mice, which may be oneof the most important antitumor mechanisms of AP.
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[基金项目]
黑龙江省科技厅攻关资助项目 (G0 0C2 0 13 0 3 );哈尔滨市科技局攻关项目 ( 2 0 0 3AA6CN0 95 )